Publications

Clinical Validation and Research

Using the Eeva™ Test adjunctively to traditional day 3 morphology is informative for consistent embryo assessment within a panel of embryologists with diverse experience

M.P. Diamond, V. Suraj, E.J. Behnke, X.Yang, M.J.Angle, J.C. Lambe Steinmiller, R. Watterson, K. Athayde Wirka, A.A. Chen, S. Shen, Journal of Assisted Reproduction and Genetics, 2014

Atypical embryo phenotypes identified by time-lapse microscopy: high prevalence and association with embryo development

K. Athayde Wirka, A.A. Chen, J. Conaghan, K. Ivani, M. Gvakharia, B. Behr, V. Suraj, L. Tan, S. Shen, Fertility and Sterility, 2014.
http://www.fertstert.org/article/S0015-0282(14)00203-9/abstract

Improving embryo selection using computer-automated time-lapse imaging plus day 3 morphology: results from a prospective multi-center trial

J. Conaghan, A.A. Chen, S.P. Willman, K.Ivani, P.E. Chenette, R.Boostanfar, V.L.Baker, G.D. Adamson, M.E. Abusief, M.Gvakharia, K.E. Loewke, S. Shen, Fertility & Sterility, 100(2): 412-9, 2013.
http://www.fertstert.org/article/S0015-0282(13)00517-7/abstract

Automated time-lapse analysis in adjunctive use with morphology is highly informative in allowing diverse embryologists to select embryos with high developmental potential

M.P. Diamond, L. Tan, J. Conaghan, K. Ivani, A. Le, A.A. Chen, S. Shen, V. Suraj, European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2014, Jun 29-Jul 2, 2014, Munich, Germany

The use of innovative, intelligent software and non-invasive embryo imaging to predict blastocyst formation by day 3

H. Marsden, J. Conaghan, K. Ivani, R. Gregoire, C. Kingsland, S. Troup
British Fertility Society/ Association of Clinical Embryologists (BFS/ACE) Annual Meeting 2013, Jan 3-5, 2013, Liverpool, UK
Awarded the “Prize Paper” for Clinical Achievement

Improved embryo selection accuracy using cell division characteristics defined by time-lapse and automated image analysis

A.A. Chen, K. Ivani, J. Conaghan, M. Gvakharia, A. Le, S. Shen
American Society for Reproductive Medicine (ASRM) Annual Meeting 2012, October 20-24, 2012, San Diego, California, Fertility & Sterility Vol.98(3):S17.

Prediction of embryo viability using validated cell division time intervals measured by time-lapse imaging

A.A. Chen, K.E. Loewke, S.P. Willman, P.E. Chenette, R. Boostanfar, S. Shen
American Society for Reproductive Medicine (ASRM) Annual Meeting 2012, October 20-24, 2012, San Diego, California, Fertility & Sterility Vol.98(3):S31.

Early cell cycle durations detected by time-lapse imaging predicts embryo developmental potential

S. Shen, A.A. Chen, S.P. Willman, P.E. Chenette, R. Boostanfar, V.L. Baker, M. Abusief, V. Suraj, K. Wirka, K. Loewke
European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2012, July 1-4, 2012, Istanbul, Turkey, Human Reproduction Vol.27(2):ii22.

Research to Advance Science

Computer-automated time-lapse analysis test results correlate to clinical pregnancy and embryo implantation: a prospective, blinded, multi-center study

M.D. VerMilyea, L. Tan, J. Anthony, J. Conaghan, K. Ivani, M. Gvakharia, R. Boostanfar, V.L. Baker, V. Suraj, A.A. Chen, M. Mainigi, C. Coutifaris, S. Shen, Reproductive Biomedicine Online, 2014

Abnormal Early Cleavage Events Predict Early Embryo Demise: Sperm Oxidative Stress and Early Abnormal Cleavage

V. Burruel, K. Klooster, C.M. Klooster, C.M. Barker, R.R. Pera, S. Meyers, Nature Scientific Reports, 2014.

Biomarkers identified with time-lapse imaging – discovery, validation and practical application

A.A. Chen, V. Suraj, R. Reijo Pera, S. Shen, Fertility and Sterility, 99:4, 1035-1043, 2013.

Computer-automated time-lapse analysis test results correlate to clinical pregnancy and embryo implantation: a prospective, blinded, multi-center study

M.D. VerMilyea, L. Tan, J. Conaghan, M. Gvakharia, K. Ivani, C. Coutifaris, P.E. Chenette, S.P. Willman, J. Anthony, M. Mainigi, V. Suraj, A.A. Chen, S. Shen, European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2014, Jun 29-Jul 2, 2014, Munich, Germany

Embryo assessment and selection by time-lapse evaluation using EEVA™ with transfer at the cleavage stage achieves similar clinical outcomes to blastocyst transfer.

M. Gaudoin, C. Adam, N. Gibson, C. Noble, P. Mitchell and R. Fleming, European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2014, Jun 29-Jul 2, 2014, Munich, Germany

Categorisation of embryo developmental potential using computerised time-lapse evaluation of early stage development reveals differences in morphology and viability between three defined categories.

C. Adam, N. Gibson, C. Noble, M. Gaudoin, P. Mitchell, R. Fleming, European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2014, Jun 29-Jul 2, 2014, Munich, Germany

Transfer of day 3 embryos selected by Morphology and Early Embryo Viability Assessment (Eeva™) show equivalent implantation rates to blastocysts

C.E. Adam, M.Gaudoin, C. Noble, N. Gibson, P. Mitchell, R. Fleming
British Fertility Society/ Association of Clinical Embryologists (BFS/ACE) Annual Meeting 2014, Jan 8-9 2014, Sheffield, UK

Using a novel time-lapse platform with automated image analysis can detect diverse abnormal cleavage patterns that are not recognized by traditional morphology and correlate with poor implantation

M. Gvakharia, K. Athayde Wirka, J. Conaghan, K. Ivani, A.A. Chen, S. Shen
American Society for Reproductive Medicine (ASRM) Annual Meeting 2013, October 12-17, Boston, Massachusetts, Fertility & Sterility Vol.100S):S463.

Adding time-lapse imaging parameters to morphology for embryo selection may improve implantation rates for cleavage-stage or blastocyst transfer

S. Vaccari, M. Gvakharia, K. Ivani, A. Le, M. Suarez, J. Conaghan
Pacific Coast Reproductive Society Annual Meeting 2013, April 17-21, 2013, Indian Wells, California, Fertility & Sterility Vol.99(3):S23-24.

Embryos exhibiting abnormal 1st cytokinesis phenotypes are associated with poorer embryo development and lower implantation potential

J. Conaghan, K. Athayde Wirka, M. Gvakharia, K. Ivani, S. Shen, A.A. Chen
American Society for Reproductive Medicine (ASRM) Annual Meeting 2013, October 12-17, Boston, Massachusetts, Published in Fertility & Sterility Vol.100(3):S90.

Early times to cavitation are associated with a reduced prevalence of aneuploidy in embryos cultured to the blastocyst stage- a prospective blinded morphokinetic study

K.H. Hong, E.J. Forman, A.Proedhel, K. Upham, R.T. Scott
American Society for Reproductive Medicine (ASRM) Annual Meeting 2013, October 12-17, Boston, Massachusetts, Fertility & Sterility Vol.100(3):S382.
Awarded Scientific Program Third Prize Poster Award.

Embryo cleavage kinetics under different oxygen tensions predict embryonic developmental potential in the mouse

R. S. Weinerman, R. Feng, T. Ord, M.D. VerMilyea, C. Coutifaris, M. Mainigi, American Society for Reproductive Medicine (ASRM) Annual Meeting 2013, October 12-17, Boston, Massachusetts, Fertility & Sterility Vol.100(3):S254.

Blastocyst development of rhesus macaque embryos produced by intracytoplasmic sperm injection (ICSI) is dependent on early cytokinesis

V. Burruel, K. Klooster, R. Reijo Pera, S. Meyers, American Society for Reproductive Medicine (ASRM) Annual Meeting 2013, October 12-17, Boston, Massachusetts, Fertility & Sterility Vol.100(3):S242-S243.

Abnormal syngamy phenotypes observed with time-lapse imaging may allow early identification of embryos with lower developmental potential

K. Athayde Wirka, V. Suraj, J. Conaghan, M. Gvakharia, K. Ivani, R. Murugesan, A.A. Chen, S. Shen, European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2013, July 7-10, 2013, London, UK, Human Reproduction Vol.28(1):i87-i90.

Dynamic assessment of early embryo fragmentation by time-lapse analysis may improve cell cycle timing-based embryo selection

J. Conaghan, L. Tan, M. Gvakharia, K. Ivani, S. Shen, R.A. Reijo Pera, A.A. Chen.
European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting 2013, July 7-10, 2013, London, UK, Human Reproduction Vol.27(2):ii344-ii348.

Non-invasive imaging for the detection of human embryonic aneuploidy at the blastocyst stage

B.E. Friedman, S.L. Chavez, B.Behr, R.B. Lathi, V.L. Baker, R.A. Reijo Pera, American Society for Reproductive Medicine (ASRM) Annual Meeting 2012, Orlando, Florida, Fertility & Sterility Vol.98(3):S38.

Embryos with good morphology but abnormal cell divisions have significantly lower implantation potential

J. Conaghan, K. Athayde Wirka, K. Ivani, A. Le, M. Gvakharia, A.A. Chen, B. Behr
Fertility Society of Australia (FSA) Annual Meeting 2012, October 28-31, 2012, Auckland, New Zealand
Awarded the Ferring “Best Scientific Paper Award”

Dynamic blastomere behavior reflects human embryo ploidy by the four-cell stage

Chavez et al., Nature Communications , 3:1251, 2012.
http://www.nature.com/ncomms/journal/v3/n12/full/ncomms2249.html

The role of time-lapse microscopy in stem cell research and therapy

Loewke et al., Stem Cells & Regenerative Medicine , Editors: K. Appasani and R. K. Appasani, Springer, 2011.
http://link.springer.com/chapter/10.1007%2F978-1-60761-860-7_11

Time-lapse microscopy and image analysis in basic and clinical embryo development research

Wong et al., Reproductive BioMedicine Online, 26(2): 120-9, 2012.
http://www.sciencedirect.com/science/article/pii/S1472648312006876

Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage

Wong et al., Nature Biotechnology, 28:10, 1115-1124, 2010.
http://www.nature.com/nbt/journal/v28/n10/abs/nbt.1686.html

Computer Vision Technology

A unified graphical models framework for automated mitosis detection in human embryos

F. Moussavi, Y. Wang, P. Lorenzen, J. Oakley, D. Russakoff, S. Gould,
IEEE Transactions in Medical Imaging, 2014.

A unified graphical models framework for automated human embryo tracking in time lapse microscopy

F. Moussavi, Y. Wang, P. Lorenzen, J. Oakley, D. Russakoff, S. Gould,
IEEE International Symposium on Biomedical Imaging, Beijing, China, 2014.

Automated embryo stage classification in time-lapse microscopy video of early human embryo development

Y. Wang, F. Moussavi, P. Lorenzen
International Conference on Medical Image Computing and Computer Assisted Intervention (MICCAI), Nagoya, Japan, 2013. Lecture Notes Computer Science Volume 8150, pp 460-467.

Development and validation of an automated computer vision algorithm for real-time embryo viability prediction

K. Loewke, F. Moussavi, M. Maddah, K. Ivani, B. Behr, V. Suraj
American Society for Reproductive Medicine (ASRM) Annual Meeting 2012, October 20-24, 2012, San Diego, California, Fertility & Sterility Vol.98(3):S288.